PTX3 ELISA Kit

Catalog No. ABIN1672819

Human PTX3 ELISA Kit Colorimetric assay for quantification of Human PTX3 and has been mentioned in 5+ publications.

Quick Overview for PTX3 ELISA Kit (ABIN1672819)

Target: PTX3 (Pentraxin 3 (PTX3))

Binding Specificity: AA 18-381

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA), Saliva

Product Details PTX3 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PTX3/Pentraxin 3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: E18-S381

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E18-S381

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: human PTX3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PTX3 has been precoated onto 96-well plates. Standards(NSO, E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PTX3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PTX3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.22, CV(%): 6.3
• Sample 2: n=16, Mean(ng/ml): 7.8, Standard deviation: 0.45, CV(%): 5.8
• Sample 3: n=16, Mean(ng/ml): 15.7, Standard deviation: 1.0, CV(%): 6.4,
• Sample 1: n=24, Mean(ng/ml): 3.86, Standard deviation: 0.28, CV(%): 7.3
• Sample 2: n=24, Mean(ng/ml): 8.1, Standard deviation: 0.48, CV(%): 5.9
• Sample 3: n=24, Mean(ng/ml): 15.3, Standard deviation: 1.16, CV(%): 7.6

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for PTX3 ELISA Kit (ABIN1672819)

Bilgin, Haliloglu, Yaman, Ay, Bilgili, Arslantas, Ture Ozdemir, Haklar, Cinel, Mulazimoglu: "Sequential Measurements of Pentraxin 3 Serum Levels in Patients with Ventilator-Associated Pneumonia: A Nested Case-Control Study." in: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale, Vol. 2018, pp. 4074169, (2018) (PubMed).

Sağ, Sağ, Tekeoğlu, Kamanli, Nas, Harman: "The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis." in: Archives of rheumatology, Vol. 33, Issue 1, pp. 39-44, (2018) (PubMed).

Kim, Won, Lee, Chun, Yoon, Kim, Kim: "Pentraxin 3 as a clinical marker in children with lower respiratory tract infection." in: Pediatric pulmonology, Vol. 51, Issue 1, pp. 42-8, (2016) (PubMed).

Patel, Shanthraj, Bhagyalakshmi, Garg, Vallakati: "Comparative evaluation of pentraxin 3 levels in GCF during canine retraction with active tieback and NiTi coil spring: An in vivo study." in: Journal of orthodontic science, Vol. 5, Issue 2, pp. 52-6, (2016) (PubMed).

Deveer, Deveer, Basaran, Turkcu, Akbaba, Cullu, Turhan, Kucuk, Kasap: "Serum Copeptin, Pentraxin 3, Anti-Mullerian Hormone Levels With Echocardiography and Carotid Artery Intima-Media Thickness in Adolescents With Polycystic Ovary Syndrome." in: Journal of clinical medicine research, Vol. 7, Issue 12, pp. 989-94, (2015) (PubMed).

Target Details for PTX3

Target: PTX3 (Pentraxin 3 (PTX3))

Alternative Name: PTX3

Background:

Protein Function: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Synonyms: Pentraxin-related protein PTX3,Pentaxin-related protein PTX3,Tumor necrosis factor alpha-induced protein 5,TNF alpha-induced protein 5,Tumor necrosis factor-inducible gene 14 protein,TSG-14,PTX3,TNFAIP5, TSG14,

Full Gene Name: Pentraxin-related protein PTX3

Cellular Localisation: Secreted.

Gene ID: 5806

UniProt: P26022